bacille calmette-guérin (bcg) vaccine  (Pasteur Institute)

Journal: Frontiers in Immunology

Article Title: C1q reprograms innate immune memory

doi: 10.3389/fimmu.2025.1515127

Figure Lengend Snippet: Single nucleotide polymorphisms in the proximity of genes encoding C1q components associate with innate immune memory responses. (A) Schematic representation of the in-vitro training experiments followed by QTL analysis using single nucleotide polymorphism (SNP) genotypes of volunteers of the 300BCG cohort. (B) Heatmap of SNPs that show a significant association with the capacity of β-glucan/BCG-induced trained immunity ( n = 267 donors). Beta shows the direction of the effect (positive = SNP increases the fold change of cytokine production upon training, and negative = SNP decreases the fold change of cytokine production). (C) Associations of SNPs rs294179, rs1561624 near C1q with the cytokine responses after 24h restimulation with LPS (10 ng/ml), 5 days after β-glucan training ( n = 267 donors). Boxplots show the genotype stratified fold changes in IL-6 and TNF responses for SNPs rs294179 and rs1561624. (D) Associations of SNPs rs666656, and rs913243 near C1q with the cytokine responses after 24h restimulation with LPS (10 ng/ml), 5 days after BCG training ( n = 267 donors). Boxplots show the genotype stratified fold changes in IL-6 and TNF responses for SNPs rs666656 and rs913243. (E) Schematic representation of the in-vivo training experiments followed by QTL analysis using SNP genotypes of volunteers of the 300BCG cohort. (F) Heatmap of SNPs that show a significant association with the capacity of the BCG vaccine-induced trained immunity ( n = 278 donors). Beta shows the direction of the effect (positive = SNP increases the fold change of cytokine production upon training, negative = SNP decreases the fold change of cytokine production) (G) Associations of SNPs rs10917276 and rs79073090 near C1q with the cytokine responses after vaccination with Bacillus Calmette–Guérin (BCG) vaccine ( n = 278 donors). Boxplots show the genotype stratified fold changes in IL-1β and TNF responses for SNPs rs10917276 and rs79073090, respectively.

Article Snippet: Individuals from the 300BCG cohort were vaccinated with 0.1 ml of BCG (BCG vaccine strain Bulgaria; Intervax, Canada).

Techniques: In Vitro, In Vivo

Journal: Frontiers in Immunology

Article Title: C1q reprograms innate immune memory

doi: 10.3389/fimmu.2025.1515127

Figure Lengend Snippet: Single nucleotide polymorphisms in the proximity of genes encoding C1q components associate with innate immune memory responses. (A) Schematic representation of the in-vitro training experiments followed by QTL analysis using single nucleotide polymorphism (SNP) genotypes of volunteers of the 300BCG cohort. (B) Heatmap of SNPs that show a significant association with the capacity of β-glucan/BCG-induced trained immunity ( n = 267 donors). Beta shows the direction of the effect (positive = SNP increases the fold change of cytokine production upon training, and negative = SNP decreases the fold change of cytokine production). (C) Associations of SNPs rs294179, rs1561624 near C1q with the cytokine responses after 24h restimulation with LPS (10 ng/ml), 5 days after β-glucan training ( n = 267 donors). Boxplots show the genotype stratified fold changes in IL-6 and TNF responses for SNPs rs294179 and rs1561624. (D) Associations of SNPs rs666656, and rs913243 near C1q with the cytokine responses after 24h restimulation with LPS (10 ng/ml), 5 days after BCG training ( n = 267 donors). Boxplots show the genotype stratified fold changes in IL-6 and TNF responses for SNPs rs666656 and rs913243. (E) Schematic representation of the in-vivo training experiments followed by QTL analysis using SNP genotypes of volunteers of the 300BCG cohort. (F) Heatmap of SNPs that show a significant association with the capacity of the BCG vaccine-induced trained immunity ( n = 278 donors). Beta shows the direction of the effect (positive = SNP increases the fold change of cytokine production upon training, negative = SNP decreases the fold change of cytokine production) (G) Associations of SNPs rs10917276 and rs79073090 near C1q with the cytokine responses after vaccination with Bacillus Calmette–Guérin (BCG) vaccine ( n = 278 donors). Boxplots show the genotype stratified fold changes in IL-1β and TNF responses for SNPs rs10917276 and rs79073090, respectively.

Article Snippet: After washing, PBMCs were incubated with culture medium only as a negative control, heat-killed Candida albicans (HKCA), 10 5 cells/ml (InvivoGen, Toulouse, France), Bacillus Calmette–Guérin (BCG) vaccine (5 μg/ml, BCG strain Bulgaria, Intervax) that acted as a positive control, or a complement factor for 24h at 37°C.

Techniques: In Vitro, In Vivo